Abundance of Escherichia coli F1-ATPase molecules observed to rotate via single-molecule microscopy with gold nanorod probes.
J Bioenerg Biomembr 39:5-6 (2007) 435-439
Abstract:
The abundance of E. coli F1-ATPase molecules observed to rotate using gold nanorods attached to the gamma-subunit was quantitated. Individual F1 molecules were determined to be rotating based upon time dependent fluctuations of red and green light scattered from the nanorods when viewed through a polarizing filter. The average number of F1 molecules observed to rotate in the presence of GTP, ATP, and without nucleotide was approximately 50, approximately 25, and approximately 4% respectively. In some experiments, the fraction of molecules observed to rotate in the presence of GTP was as high as 65%. These data indicate that rotational measurements made using gold nanorods provide information of the F1-ATPase mechanism that is representative of the characteristics of the enzyme population as a whole.Recent developments of bio-molecular motors as on-chip devices using single molecule techniques.
Lab Chip 7:12 (2007) 1633-1643
Abstract:
The integration of microfluidic devices with single molecule motor detection techniques allows chip based devices to reach sensitivity levels previously unattainable.A functionally inactive, cold-stabilized form of the Escherichia coli F1Fo ATP synthase
Biochimica et Biophysica Acta - Bioenergetics 1757:3 (2006) 206-214
Structure and function of subunit a of the ATP synthase of Escherichia coli
Journal of Bioenergetics and Biomembranes 37:6 (2005) 445-449
Ultrafast purification and reconstitution of His-tagged cysteine-less Escherichia coli F 1F o ATP synthase
Biochimica et Biophysica Acta - Bioenergetics 1706:1-2 (2005) 110-116