Publications associated with Single Molecule Spectroscopy of Gene Machines

Aligning the μs-ALEX Setup.

Cold Spring Harbor protocols 2015 (2015) 1027-1028

A Kapanidis, D Majumdar, M Heilemann, E Nir, S Weiss

To achieve single-molecule sensitivity and thus have the ability to detect single diffusing fluorophores, careful alignment of the microsecond-alternating laser excitation (μs-Alex) setup is crucial. The following protocol describes routine alignment for 2c-ALEX (532 nm/635 nm) with spectral windows G(550-620)R(650-750).

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