Interferometric scattering microscopy: From high-speed nanometry to ultra-sensitive label-free imaging

Philipp Kukura (Oxford)

I will highlight the capabilities of an alternative approach to optical microscopy that relies on the detection of light scattering called interferometric scattering microscopy (iSCAT). I will illustrate how the unique combination of nm precision and microsecond time resolution can be used to address fundamental questions about the processivity of molecular motors or the diffusion of receptors in bilayer membranes. In addition, I will show how iSCAT can detect, image and track the motion of single proteins without the need for any labels and how this sensitivity can be used to study phenomena ranging from lipid nanodomains in model membranes to micelle and vesicle dynamics relevant to pre-biotic chemistry and the origin of life.