DNA-based optical sensors for forces in cytoskeletal networks
ACS Applied Nano Materials American Chemical Society 6:17 (2023) 15455-15464
Abstract:
Mechanical forces are relevant for many biological processes, from wound healing and tumor formation to cell migration and differentiation. Cytoskeletal actin is largely responsible for responding to forces and transmitting them in cells, while also maintaining cell shape and integrity. Here, we describe a FRET-based hybrid DNA-protein tension sensor that is designed to sample transient forces in actin networks by employing two actin-binding motifs with a fast off-rate attached to a central DNA hairpin loop. Such a sensor will be useful to monitor rapidly changing stresses in the cell cytoskeleton. We use fluorescence lifetime imaging to determine the FRET efficiency and thereby the conformational state of the sensor, which makes the measurement robust against intensity variations. We demonstrate the applicability of the sensor by confocal microscopy and by monitoring crosslinking activity in in vitro actin networks by bulk rheology.Reconfigurable self-assembled DNA devices
Science Robotics American Association for the Advancement of Science 8:77 (2023) eadh8148
Abstract:
Modular reconfigurable systems can be achieved with DNA origami, demonstrating the potential to generate molecular robots.
A modular RNA delivery system comprising spherical nucleic acids built on endosome-escaping polymeric nanoparticles
Nanoscale Advances Royal Society of Chemistry (RSC) (2023)
Abstract:
<jats:p>Polymeric spherical nucleic acids comprise pH-sensitive, polymer-conjugated oligonucleotides that self-assemble into nanoparticles with the ability to escape endosomes, overcoming a major obstacle in nucleic acid delivery.</jats:p>DNA-PAINT microscope data of a DNA nanostructure printer
University of Oxford (2022)
Abstract:
This dataset consist of reconstructed DNA-PAINT images of DNA origami based molecular devices. This is the data from the paper "A DNA molecular printer capable of programmable positioning and patterning in two dimensions". The data is structures after the figure of the paper. It is reconstructed and can be opened using the DNA-PAINT software Picasso. The data is described by what DNA paint probe was used to image it, corresponding to multiple image channels. 'P1' is the DNA-PAINT docking handle used on the frame and the canvas, 'R1' is the DNA-PAINT docking handle used on the sleeve, and 'R3' is the DNA-PAINT docking handle used on the ink patterned on the canvas.Strategies for constructing and operating DNA origami linear actuators
Small Wiley 17:20 (2021) 2007704