Publications associated with Self-assembled structures and devices

DNA-templated protein arrays for single-molecule imaging.

Nano Lett 11 (2011) 657-660

DN Selmi, RJ Adamson, H Attrill, AD Goddard, RJ Gilbert, A Watts, AJ Turberfield

Single-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show that self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this technique using a G-protein-coupled membrane receptor, a soluble G-protein, and a signaling complex of both molecules.

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